Optimized fermentation processes for the preparation of...

Details Optimized fermentation processes for the preparation of... Optimized fermentation processes for the preparation of...

C - Chemistry, Metallurgy

12

P

195/1.2, 195/1.2

C12P 21/00 (2006.01) C07K 14/47 (2006.01) C12N 15/70 (2006.01) C12N 15/72 (2006.01)

Patent

CA 2022487

The invention describes optimized fermentation processes for the preparation of foreign proteins in E.coli using the lac promoter or improved lac promoter (for example tac, trc). After the initial growth phase with glucose as carbon source, induction of product formation is effected (1) via IPTG with glucose limitation or (2) via lactose with lactose limitation or (3) via IPTG and lactose with lactose limitation. The limitation of glucose or lactose is such that the oxygen partial pressure remains above 10%.

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