C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/82 (2006.01) A01H 1/00 (2006.01) A61K 48/00 (2006.01) C12N 15/80 (2006.01) C12N 15/81 (2006.01)
Patent
CA 2394840
The present invention relates to T-DNA vectors and methods for obtaining transgenic eukaryotes using said vectors. The transgenic eukaryotes are characterized in that they contain the T-DNA but not the illegitimately transferred vector backbone sequence. This is achieved by modifying the T-DNA borders such that they are more efficiently nicked or such that they allow elimination of illegitimately transferred vector backbone sequences by means of recombination.
La présente invention se rapporte à des vecteurs d'ADN-T et à des méthodes de production d'eucaryotes transgéniques à partir desdits vecteurs. Les eucaryotes transgéniques se caractérisent en ce qu'ils contiennent l'ADN-T mais pas la séquence squelette du vecteur transféré illégitimement. Ceci résulte d'une modification des frontières de l'ADN-T de sorte que celles-ci soit plus efficacement entaillées ou de sorte qu'elles permettent l'élimination des séquences squelette du vecteur transféré illégitimement par recombinaison.
Broekaert Willem
de Buck Sylvie
de Wilde Chris
Depicker Anna
Mironov Vladimir
Cropdesign N.v.
Smart & Biggar
LandOfFree
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