Osteogenic factors

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

167/327, 530/15.

C12N 15/18 (2006.01) A61K 38/18 (2006.01) A61L 27/22 (2006.01) C07K 14/51 (2006.01) C07K 16/22 (2006.01) C12N 5/10 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2000498

The present invention provides an osteogenically active protein preparation characterized by a molecular weight of from about 31,000 to 34,000 daltons as characterized by non-reducing sodium dodecyl sulfate polyacrylamide gel electrophoresis and by the characteristic of eluting from a reverse phase high performance liquid chromatography column equilibrated with buffers containing trifluoroacetic acid and acetonitrile by eluting within the concentrations of 35~ to 45% acetonitrile. The invention further provides improved methods for isolating such preparations and genes encoding all or a portion of polypeptide subunits of dimers comprising the osteogenic protein preparation.

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