C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 21/06 (2006.01) C12N 9/16 (2006.01) C12Q 1/42 (2006.01)
Patent
CA 2516794
Compositions for an alkaline phosphatase and methods for over-expression and purification of thermolabile Antarctic phosphatase (TAP) are provided. Uses for TAP include dephosphorylation of nucleic acids, sugars, peptides and proteins. TAP as described herein has advantages over phosphatases from other sources with respect to thermolability at 65~C and efficiency of dephosphorylation activity at approximately neutral pH.
L'invention concerne des compositions destinées à une phosphatas alcaline et des méthodes de surexpression et de purification de la phosphatase thermolabile <=Antarctic>= (TAP). L'utilisation de TAP consiste à déphosphoryler des acides nucléiques, des sucres, des peptides et des protéines. TAP telle que décrite ci-dessus présente des avantages par rapport aux phosphatases provenant d'autres sources concernant la thermolabilité à 65 ·C et l'efficacité de l'activité de déphosphorylation à un pH approximativement neutre.
Benner Jack II
Davis Theodore
Guthrie Ellen
New England Biolabs Inc.
Ridout & Maybee Llp
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