C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 7/40 (2006.01) C12N 9/04 (2006.01) C12N 9/08 (2006.01)
Patent
CA 2127094
A process for the production of Glyoxylic acid from glycolic acid where an aqueous solution of about 200 to 2500 mM gly- colic acid at a pH of 7 to 10, at 0° to 40 °C is contacted with an effective amount of whole microbial cells (e.g., Aspergillus nidu- lans. Pichia pastoris, Hansenula polymorpha and Escherichia coli) which as a result of genetic engineering expresses the enzyme glycolate oxidase, (S)-2-hydroxy-acid oxidase (EC 1.1.3.15), to achieve an effective concentration range of 0.01 to about 100 IU/ mL and optionally expresses an endogenous catalase (EC 1.11.1.6).
Anton David L.
Dicosimo Robert
Dimock Stratton Llp
E. I. Du Pont de Nemours And Company
University Of Iowa Research Foundation
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