Oxidizing phenolic compounds in enzymatic immunoassay

G - Physics – 01 – N

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G01N 33/554 (2006.01) G01N 33/53 (2006.01) G01N 33/535 (2006.01) G01N 33/58 (2006.01)

Patent

CA 1104928

ABSTRACT: An assay for the quantitative determination of entities selected from immunocens and antibodies, which comprises: a. preparing antibodies specific to the entity to be determined, b. preparing an antigen-peroxidase conjugate or an antibody-peroxidase conjugate specific to said entity, c. preparing a system containing a quantity of peroxidase- tagged antigen or peroxidase-tagged antibody proportional to the quantity of the entity which is being determined. d. oxidizing a phenolic compound emitting light when subjected to enzymatic oxidation by means of a peroxy- compound in the presence of the tagged substance. e. measuring the light emitted and thus establishing the quantity of the said entity, a kit for carrying out such assay and a device for the quantitative determination of immunogens and antibodies by the above assay.

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