Oxidoreductase from filamentous fungi, dna coding therefor...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/53 (2006.01) C12N 1/15 (2006.01) C12N 9/02 (2006.01) C12N 15/80 (2006.01) C12P 1/02 (2006.01)

Patent

CA 2164206

Disclosed is a new gene which codes for a NADPH cytochrome P450 oxidoreductase from filamentous fungi. Also disclosed are a recombinant DNA molecule which comprises at least a part of that gene, an RNA molecule derived therefrom and a new polypetide (or protein), as well as at least one host cell transformed with such recombinant DNA molecule. Particularly suitable host cells are those originating from a filamentous fungus. With the polypeptides (proteins) and the host cells according to the invention, new processes for enzymatic conversions can be performed. In particular, enzymatic conversions by meaus of monooxygenases, more particularly by means of enzymes from the cytochrome P450 superfamily are improved with the invention.

Est décrit un nouveau gène qui code pour une NADPH cytochrome P450 oxydoréductase issue de champignons filamenteux. Sont également décrites une molécule d'ADN recombiné qui comporte au moins une partie de ce gène, une molécule d'ARN dérivée de celle-ci et un nouveau polypeptide (ou protéine), ainsi qu'au moins une cellule hôte transformée avec ladite molécule d'ADN recombiné. Les cellules hôtes issues d'un champignon filamenteux sont particulièrement appropriées. Les polypeptides (protéines) et les cellules hôtes décrites permettent d'exécuter de nouveaux procédés de conversion enzymatique. En particulier, l'invention permet de perfectionner les conversions enzymatiques à l'aide de monooxygénases, plus particulièrement à l'aide d'enzymes issues de la superfamille des cytochromes P450.

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