Oxidoreductase from pichia capsulata

Details Oxidoreductase from pichia capsulata Oxidoreductase from pichia capsulata

C - Chemistry, Metallurgy

12

N

C12N 15/53 (2006.01) C07K 16/40 (2006.01) C07K 19/00 (2006.01) C12N 9/02 (2006.01) C12P 7/02 (2006.01) C12P 41/00 (2006.01)

Patent

CA 2529583

The invention relates to a NADH-dependent oxidoreductase obtained from yeasts, for example, of the genus Pichia, more particularly from Pichia capsulata. Said oxidoreductase in then used in an enzymatic process for enantioselective reduction of organic ketocompounds to the corresponding (S)-hydroxy compounds and in an enzymatic process for enantioselective preparation of (S)-hydroxy compounds in a two phase system using the isolated, recombinantly overexpressed oxidoreductase from Pichia capsulata.

Selon l'invention, une oxydoréductase dépendant du NADH est extraite de levures, par exemple du genre Pichia, notamment du genre Pichia capsulata, est utilisée dans un procédé enzymatique servant à la réduction énantiosélective de composés cétoniques organiques en composés (S)-hydroxy correspondants et dans un procédé enzymatique servant à l'obtention énantiosélective de composés (S)-hydroxy dans un système à deux phases au moyen de l'oxydoréductase surexprimée recombinée isolée, extraite de Pichia capsulata.

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