Oxygen scavenging system for anaerobiosis

C - Chemistry – Metallurgy – 12 – M

Patent

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217/24

C12M 1/22 (2006.01) C12M 1/04 (2006.01)

Patent

CA 1228576

OXYGEN SCAVENGING SYSTEM FOR ANAEROBIOSIS ABSTRACT OF THE DISCLOSURE A self-contained system is provided for producing an oxygen-free environment for anaerobiosis and cul- turing wherein the system possesses the ability to pro- vide and maintain the oxygen-free environment, even after the opening and closing of the sealed container therefore several times. The system includes a metal- lic couple, preferably copper-magnesium, which reacts with water to induce and maintain the oxygen-free en- vironment. The degree of addition of water to the sys- tem controls the rate of reduction of the environment. The system possesses a dual oxygen scavenging function by the controlled addition of water. That is, if a small amount of moisture is added to the system couple of the invention, hydrogen is liberated, which causes a drying in the system simultaneously with a reaction of a portion of the couple to gradually scavenge oxy- gen from the environment, simultaneously with the liber- ation of hydrogen. If desired, in such an arrangement the subsequent addition of water generates hydrogen again until all the magnesium is used up, in order to provide a subsequent oxygen scavenging property. In addition, the system includes a sealable petri dish so conformed to include means for maintaining the sys- tem of the invention in combination with an indicator for anaerobiosis, a catalyst for the system, a carbon dioxide generator, and space for the culture media.

403429

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