C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/53 (2006.01) A01H 1/00 (2006.01) A01H 1/04 (2006.01) A01H 5/00 (2006.01) C12N 9/02 (2006.01) C12N 15/82 (2006.01) C12P 13/04 (2006.01)
Patent
CA 2396375
The invention provides DNA coding for cytochrome P450 monooxygenases of the CYP79 family catalyzing the conversion of an aliphatic or aromatic acid or chain-elongated methionine homologue to the corresponding oxime. Preferred embodiments of the invention are enzymes catalyzing the conversion of L-Valine and L-Isoleucine such as the cassava enzymes CYP79D1 and CYP79D2, enzymes catalyzing the conversion of tyrosine such as the Triglochin maritima enzymes CYP79E1 and CYP79E2, enzymes catalyzing the conversion of tryptophan to the corresponding oxime indole-3-acetaldoxine such as the Arabidopsis thaliana enzyme CYP79A2 and the Bassica napus enzyme CYP79B5, and enzymes catalyzing the conversion of a chain-elongated methionine homologue such as theArabidopsis thaliana enzymes CYP79F1 and CYP79F2. Transgenic expression of said DNA or parts thereof in plants can be used to manipulate the biosynthesis of corresponding glucosinolates or cyanogenic glucosides.
La présente invention concerne de l'ADN codant pour des monooxygénases P450 cytochromes de la famille CYP79 catalysant la conversion d'un acide aminé aliphatique ou aromatique ou d'un homologue méthionine à longue chaîne en l'oxime correspondante. Des modes de réalisation préférés de l'invention concernent des enzymes catalysant la conversion de valine L et isoleucine L telles que les enzymes cassava CYP79D1 et CYP79D2, des enzymes catalysant la conversion de la tyrosine telles que les enzymes Triglochin maritima CYP79E1 et CYP79E2, des enzymes catalysant la conversion du tryptophane en l'oxime correspondante indole-3-acétaldoxime telles que l'enzyme Arabidopsis thaliana CYP79A2 et l'enzyme Bassica napus CYP79B5, et des enzymes catalysant la conversion de l'homologue méthionine à longue chaîne telles que les enzymes Arabidopsis thaliana CYP79F1 et CYP79F2. L'expression transgénique dudit ADN ou de parties de celui-ci dans des végétaux peut être utilisée pour agir sur la biosynthèse des glucosinolates ou glucosides cyanogéniques correspondants.
Andersen Mette Dahl
Bak Soren
Busk Peter Kamp
Halkier Barbara Ann
Hansen Carsten Horslev
Fetherstonhaugh & Co.
Royal Veterinary And Agricultural University
Syngenta Participations Ag
LandOfFree
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