Packed bed for nucleic acid capture and amplification

Details Packed bed for nucleic acid capture and amplification Packed bed for nucleic acid capture and amplification

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2602366

A system for nucleic acid capture and amplification comprising introducing a sample potentially containing the nucleic acid into a packed bed wherein the nucleic acid adheres to the packed bed, introducing an amplification mix into the packed bed/ and thermal cycling the packed bed and the nucleic acid between denaturation and annealing temperatures for PCR amplification. One embodiment provides an apparatus for DNA capture and amplification comprising a tubing or housing having a cavity, bed media in the cavity, and a heater operatively connected to the tubing or housing.

L'invention concerne un système de capture et d'amplification d'acide nucléique qui consiste à introduire un échantillon contenant potentiellement l'acide nucléique dans un lit granulaire fixe, l'acide nucléique adhérant au lit granulaire fixe, à introduire un mélange d'amplification dans le lit granulaire fixe et à procéder au cyclage thermique du lit granulaire et de l'acide nucléique entre les températures de dénaturation et d'annelage afin d'effectuer l'amplification en chaîne par polymérase (PCR). Dans un mode de réalisation, l'invention concerne un appareil de capture et d'amplification d'ADN comprenant un tube ou un logement comportant une cavité, un milieu de lit dans la cavité, et un élément chauffant fonctionnellement relié au tube ou au logement.

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