Paired end sequencing

Details Paired end sequencing Paired end sequencing

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2615323

The present invention provides for a method of preparing a target nucleic acid fragments to produce a smaller nucleic acid which comprises the two ends of the target nucleic acid. Specifically, the invention provides cloning and DNA manipulation strategies to isolate the two ends of a large target nucleic acid into a single small DNA construct for rapid cloning, sequencing, or amplification.

La présente invention concerne un procédé de préparation de fragments d'acides nucléiques cibles en vue de produire un acide nucléique plus petit qui comporte les deux extrémités de l'acide nucléique cible. L'invention concerne en particulier des stratégies de clonage et de manipulation d'ADN visant à isoler les deux extrémités d'un grand acide nucléique cible en une seule petite construction d'ADN en vue d'un clonage, d'un séquençage ou d'une amplification rapide.

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