C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) G06F 19/00 (2006.01)
Patent
CA 2627259
Method of preparing a calibration curve and determining the amount of an analyte polynucleotide present in a test sample, particularly using real-time amplification data. The method involves determining indicia of amplification for both the internal calibrator and analyte polynucleotide standards, each as a function of the amount of analyte polynucleotide standard input into the amplification reaction.
L~invention concerne un procédé de préparation d~une courbe d~étalonnage et de détermination de la quantité d~un polynucléotide analyte présente dans un échantillon de test, particulièrement par l~exploitation de données d~amplification en temps réel. Le procédé implique la détermination d~un indice d~amplification à la fois pour l~étalonneur interne et pour des critères de polynucléotides analytes, chacun étant fonction de la quantité de polynucléotide analyte introduite de façon standard dans la réaction d~amplification.
Carrick James M.
Chismar Jeffrey D.
Kennedy Mark R.
Gen-Probe Incorporated
Smart & Biggar
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