Paramyxovirus-derived rnp

Details Paramyxovirus-derived rnp Paramyxovirus-derived rnp

C - Chemistry, Metallurgy

12

N

C12N 15/45 (2006.01) C07K 14/115 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2368944

A functional RNP containing negative-strand single-stranded RNA derived from Sendai virus, which has been modified so as not to express any envelope protein, has been successfully prepared. An RNP comprising a foreign gene is prepared and inserted into a cell with the use of a cationic liposome, thereby successfully expressing the foreign gene.

L'invention se rapporte à une ribonucléoprotéine (RNP) contenant de l'ARN simple brin à chaîne (-) issu du virus Sendai, qui a été modifiée de manière à n'exprimer aucune protéine enveloppe. On prépare une RNP contenant ce gène étranger et on l'introduit dans une cellule à l'aide d'un liposome cationique, ce qui permet d'exprimer avec succès le gène étranger.

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