C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/0735 (2010.01) C12N 5/071 (2010.01) C12N 5/075 (2010.01) C40B 40/02 (2006.01)
Patent
CA 2626642
Methods of producing human stem cells are disclosed for parthenogenetically activating human oocytes by manipulation of O2 tension, including manipulation of Ca2+ under high O2 tension and contacting oocytes with serine threonine kinase inhibitors under low O2 tension, isolating inner cell masses (ICMs) from the activated oocytes, and culturing the cells of the isolated ICMs under high O2 tension. Moreover, methods are described for the production of stems cells from activated oocytes in the absence of non-human animal products, including the use of human feeder cells/products for culturing ICM/stem cells. Stem cells produced by the disclosed methods are also described.
L~invention concerne des méthodes pour produire des cellules souches humaines dans le but d~activer parthénogénétiquement des oocytes humains en manipulant la pression d~O2, consistant à manipuler des ions Ca2+ sous haute pression d~O2 et à mettre en contact des oocytes avec des inhibiteurs de la sérine thréonine kinase sous basse pression d~O2, à isoler des masses cellulaires internes (MCI) à partir d'oocytes activés et à cultiver les cellules MCI isolées sous haute pression d~O2. De plus, l'invention concerne des méthodes pour la production de cellules souches d'oocytes activés en l~absence de produits animaux non humains, comprenant l'utilisation de cellules/produits nourriciers humains pour cultiver des MCI/cellules souches. L'invention concerne également les cellules souches produites par lesdites méthodes.
Janus Jeffrey D.
Kuzmichev Leonid N.
Pryzhkova Marina V.
Revazova Elena S.
International Stem Cell Corporation
Mbm Intellectual Property Law Llp
LandOfFree
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