Partially purified osteogenic factor and process for...

Details Partially purified osteogenic factor and process for... Partially purified osteogenic factor and process for...

A - Human Necessities

61

K

167/104

A61K 35/32 (2006.01) A61L 27/22 (2006.01) C07K 14/51 (2006.01) A61K 38/00 (2006.01)

Patent

CA 1223199

Abstract A process for partially purifying an osteogenic factor from demineralized bone particles or osteosarcomas in which nonfibrous proteins are extracted from the demineralized bone or osteosarcomas with a dissociative extractant for nonfibrous proteins, such as urea or guanidine hydrochloride, the extracted nonfibrous proteins are fractionated by anion exchange chromatography using DEAE cellulose at pH 7, the fraction not adsorbed by the DEAE cellulose is further fractionated by cation exchange chromatography using CM cellulose at pH 4.8, the fraction adsorbed by the CM cellulose is eluted therefrom and the partially purified osteogenic factor is recovered from the eluate as a ?30,000 dalton protein isolate.

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