Particle bombardment transformation of brassica

Details Particle bombardment transformation of brassica Particle bombardment transformation of brassica

C - Chemistry, Metallurgy

12

N

C12N 5/10 (2006.01) A01H 5/00 (2006.01) C12N 5/04 (2006.01) C12N 15/29 (2006.01) C12N 15/90 (2006.01)

Patent

CA 2440416

The invention involves methods and materials related to the transformation of Brassica by particle bombardment. Specifically, the invention provides methods of preparing non-embryo Brassica tissue such that Brassica cells are capable of being cultured, transformed by particle bombardment, and regenerated into plants. In addition, this invention provides stably transformed Brassica cells as well as their progeny. This invention also provides methods of culturing Brassica tissue with liquid medium such that transformed Brassica cells are identified and regenerated into transformed Brassica plants.

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