Particle-mediated transformation of gymnosperms

Details Particle-mediated transformation of gymnosperms Particle-mediated transformation of gymnosperms

C - Chemistry, Metallurgy

12

N

C12N 5/10 (2006.01) A01H 4/00 (2006.01) A01H 7/00 (2006.01) C12N 15/82 (2006.01) C12N 15/90 (2006.01)

Patent

CA 2075135

ABSTRACT OF THE DISCLOSURE A method for genetically engineering gymnosperm tree species is based on accelerated particle transformation. Tissue of spruce is cultured into an embryogenic callus. Somatic embryos are produced by the callus and then subjected to an accelerated particle transformation process. The treated embryos are then induced to form embryogenic callus cultures and selected for the presence of gene products coded by the introduced genes. Somatic embryos are then induced from the callus and transgenic seedlings are produced.

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