Particle-mediated transformation of mammalian unattached cells

Details Particle-mediated transformation of mammalian unattached cells Particle-mediated transformation of mammalian unattached cells

C - Chemistry, Metallurgy

12

N

C12N 15/87 (2006.01) C12N 5/10 (2006.01)

Patent

CA 2098498

2098498 9308270 PCTABS00021 A method of genetically transforming mammalian unattached cells is disclosed. The method begins by preparing copies of a nucleic acid construct and coating these copies onto biologically inert carrier particles. Mammalian unattached cells are isolated in a liquid suspension. The cell suspension is placed on a target surface, wherein the liquid is spread to a thin film on the target surface. In an alternative embodiment of the present invention, the liquid is spread onto a porous surface. The cells are bombarded with the construct-coated particles in such a fashion that some particles lodge in the interior of at least some of the cells. The existence and expression of the construct within the cell is verified.

Affiliated with

Also associated with

Rating

Particle-mediated transformation of mammalian unattached cells does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Particle-mediated transformation of mammalian unattached cells, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Particle-mediated transformation of mammalian unattached cells will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-2067590