Particle transfection: a method for the transfer of...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/87 (2006.01)

Patent

CA 2146971

A method of directly transfecting a large number of eukaryotic, prokaryotic or plant cells, which retains substantial cell vi- ability is achieved by the present invention. The method includes the steps of contacting with cells adhered to a support, an amount of polynucleotide molecule targeted for transfection into the cells and an amount of particles. A gentle agitation of the cells, polynucleotide molecules and particules permits direct transfection of the polynucleotide molecules into the cells.

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