Particle with luminescer for assays

Details Particle with luminescer for assays Particle with luminescer for assays

G - Physics

01

N

167/43

G01N 33/58 (2006.01) C09B 23/01 (2006.01) C09B 57/00 (2006.01) G01N 33/537 (2006.01) G01N 33/542 (2006.01) G01N 33/543 (2006.01)

Patent

CA 1312822

25870 ABSTRACT OF THE DISCLOSURE Assay methods are provided for determining an analyte in a sample suspected of containing the analyte. The method is carried out using a composition that includes a conjugate of a first sbp member with a particle. A luminescer is reversibly associated with a nonaqueous phase of the particle. Where the first sbp member is not complementary to the analyte, a second sbp member that is capable of binding to the first sbp member is employed. Unbound conjugate is separated from conjugate that is bound to the analyte or to the second sbp member. A reagent for enhancing the detectability of the luminescer is added and the light emission of the luminescer acted on by the reagent is measured. 6831H 25870-FF

555881

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