Pathways arrays

G - Physics – 01 – N

Patent

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Details

G01N 33/53 (2006.01) B01J 19/00 (2006.01) C07B 61/00 (2006.01) G01N 33/543 (2006.01) G01N 33/551 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2419544

The present invention provides array systems that facilitate the simultaneous monitoring of many interactions between biological molecules. Biomolecule arrays according to the present invention provide a flexible and general tool and will alloy biological researchers to probe cellular protein interactions with high throughput. In certain embodiments, the present invention provides methods and arrays for analyzing biochemical pathways comprising the steps of forming an array of immobilized biomolecules; exposing said array to biomolecules in solution; and detecting modification of the immobilized biomolecules, modification of the biomolecules in solution, and/or binding of biomolecules in solution to immobilized biomolecules.

La présente invention concerne des systèmes de réseaux permettant de simplifier la surveillance simultanée de plusieurs interactions entre des molécules biologiques. Les réseaux biomoléculaires selon l'invention représentent un outil flexible et général permettant à des chercheurs en biologie de sonder des interactions de protéines cellulaires avec un débit élevé. Dans certains modes de réalisation, la présente invention concerne des procédés et des réseaux destinés à analyser des voies biochimiques. Ledit procédé consiste à former un réseau de biomolécules immobilisées, à exposer ledit réseau à des biomolécules en solution, et à détecter une modification des biomolécules immobilisées, une modification des biomolécules en solution, et/ou une liaison de biomolécules en solution à des biomolécules immobilisées.

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