Patterned surfaces with chemical crosslinkers for use in...

G - Physics – 01 – N

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G01N 33/543 (2006.01) C12Q 1/68 (2006.01) G01N 21/47 (2006.01)

Patent

CA 2577140

Fabrication of surfaces patterned with chemical crosslinkers for solution- phase immobilization of probe molecules and their use in diffraction-based sensing. In one embodiment of the invention, a chemical crosslinker, X1 - R1 - Y1, is deposited on areas of the substrate surface that defines a pattern and allowed to react with the surface for a sufficient period of time to attain the desired density of covalently linked crosslinkers on the surface. The reaction between the crosslinker X1 - R1 - Y1 and the surface can be accelerated using known techniques such as heating, microwave irradiation, sonication, etc, to achieve the desired density in less time. In another embodiment of the invention, two or more other types of cross-linkers may also be laid down in patterns on the surface to detect for two or more other types of molecules in solution.

L'invention concerne la fabrication de surfaces configurées par des agents de réticulation chimiques afin d'effectuer l'immobilisation en phase de solution de sondes moléculaires, et leur utilisation en détection à base de diffraction. Dans un mode de réalisation, on dépose un agent de réticulation chimique, X1 - R1 -Y1, sur des zones de la surface du substrat définissant une configuration et on laisse la réaction s'effectuer avec la surface pendant une durée suffisante pour obtenir la densité désirée d'agents de réticulation possédant des liaisons covalentes sur la surface. On peut accélérer la réaction entre l'agent de réticulation X1 - R1 - Y1 et la surface au moyen de techniques connues, telles que réchauffement, irradiation micro-ondes, sonication, par exemple, de manière à atteindre la densité désirée en un laps de temps limité. Dans un autre mode de réalisation, on peut également déposer sous forme de motifs sur la surface deux ou plusieurs autres types d'agent de réticulation afin de détecter deux ou plusieurs autres types de molécules en solution.

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