Pca3 messenger rna species in benign and malignant prostate...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/12 (2006.01) A01K 67/027 (2006.01) C07K 14/47 (2006.01) C07K 16/18 (2006.01) C12Q 1/68 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2385477

This invention concerns the discovery of two distinct PCA3 mRNA sequences. One of these sequences corresponds to a short PCA3 mRNA mole-cule whereas the other PCA3 RNA molecule is longer as it comprises an additional sequence between exon 3 and exon 4a. The short RNA is associated with prostate cancer whereas the long RNA sequence is associated with a non-malignant state of the prostate. Based on the differential expression levels of these two PCA3 RNA sequences, protocols for the diagnosis of prostate dis-ease are provided. The invention also relates to thera-peutic approaches to prostate cancer.

L'invention porte sur la découverte de deux séquences distinctes de l'ARNm du PCA3 dont l'une correspond à une molécule courte de l'ARNm du PCA3, et l'autre à une molécule plus longue en ce qu'elle comporte une séquence additionnelle comprise entre l'exon 3 et l'exon 4. L'ARN court est associé au cancer de la prostate, tandis que l'ARN long l'est à des troubles non malins de la prostate. On a ainsi pu établir des protocoles de diagnostic des pathologies de la prostate basés sur les niveaux différentiels d'expression des deux séquences de l'ARNm du PCA3.

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