Pcka modifications and enhanced protein expression in bacillus

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/60 (2006.01) C12N 9/00 (2006.01) C12N 9/02 (2006.01) C12N 9/10 (2006.01) C12N 9/52 (2006.01) C12N 9/88 (2006.01) C12N 15/09 (2006.01) C12N 15/31 (2006.01) C12N 15/52 (2006.01) C12N 15/53 (2006.01) C12N 15/54 (2006.01) C12N 15/55 (2006.01) C12N 15/70 (2006.01) C12N 15/74 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2562208

The present invention provides cells that have been genetically manipulated to have an altered capacity to produce expressed proteins, wherein the pckA gene has been modified or deleted. In particular, the present invention relates to Gram-positive microorganisms, such as Bacillus species having enhanced expression of a protein of interest, wherein one or more chromosomal genes have been modified and/or inactivated (e.g., pckA), and preferably wherein one or more chromosomal genes (e.g., pckA) have been modified and/or deleted from the Bacillus chromosome. In some further embodiments, one or more indigenous chromosomal regions have been modified and/or deleted from a corresponding wild-type Bacillus host chromosome.

La présente invention concerne des cellules qui ont été génétiquement manipulées afin de présenter une capacité modifiée à produire des protéines exprimées, le gène pckA ayant été modifié ou supprimé. Cette invention concerne en particulier des micro-organismes Gram positifs, tels qu'une espèce Bacillus présentant une expression améliorée d'une protéine cible, dans lesquels un ou plusieurs gènes chromosomiques ont été modifiés ou inactivés (par ex. pckA), de préférence dans lesquels un ou plusieurs gènes chromosomiques (par ex. pckA) ont été modifiés ou supprimés du chromosome de Bacillus. Dans d'autres modes de réalisation, une ou plusieurs régions chromosomiques indigènes ont été modifiées et/ou supprimées d'un chromosome hôte deBacillus de type sauvage correspondant.

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