Pcr amplification of rearranged genomic variable regions of...

Details Pcr amplification of rearranged genomic variable regions of... Pcr amplification of rearranged genomic variable regions of...

C - Chemistry, Metallurgy

12

N

C12N 15/13 (2006.01) C07K 16/00 (2006.01) C07K 16/10 (2006.01) C07K 16/46 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2206534

The invention features methods for isolating immunoglobulin variable region genes, and the use of these genes in the production of chimeric and isotype switched antibodies. The invention also features substantially pure DNA encoding a variable region of the antibody produced by hybridoma cell line HNK- 20, and chimeric antibodies containing this variable region.

Techniques d'isolation des gènes à région variable de l'immunoglobuline et utilisation de ces gènes pour produire des anticorps chimères et à commutation isotypique. L'invention porte également sur de l'ADN sensiblement pur codant une région variable de l'anticorps produit par la lignée cellulaire de l'hybridome HNK-20 et sur des anticorps chimères contenant cette région variable.

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