Pcr-based detection and quantification of tapesia yallundae...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2381204

The present invention provides primers and probes for use in TaqManTM quantitative PCR assays for the detection of Tapesia yallundae (syn. Pseudocercosporella herpotrichoides W-type) and Tapesia acuformis (syn. Pseudocercosporella herpotrichoides R-type). The present invention also provides primers and probes for use in TaqManTM quantitative PCR control assays for the detection of wheat DNA.

L'invention concerne des amorceurs et des sondes à utiliser dans des dosages TaqMan?TM¿ quantitatifs de PCR permettant de détecter Tapesia yallundae (syn. Pseudocercosporella herpotrichoides type W) et Tapesia acuformis (syn. Pseudocercosporella herpotrichoides type R). L'invention traite également d'amorceurs et de sondes à utiliser dans des dosages de contrôle TaqMan?TM¿ quantitatifs pour la détection de l'ADN du blé.

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