Pcr-based detection of rhizoctonia cerealis

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07K 14/37 (2006.01) C12N 15/31 (2006.01)

Patent

CA 2395233

Internal Transcribed Spacer (ITS) DNA sequences from the ribosomal RNA gene region are described for different strains of the wheat fungal pathogen, Rhizoctonia cerealis. Specific primers from within these sequences are identified as being useful for the identification of Rhizoctonia cerealis using PCR-based techniques.

L'invention concerne des séquences ADN espaceur transcrites internes (ITS) de la région génique d'ARN ribosomal concernant différentes souches du pathogène fongique du blé, Rhizoctonia cerealis. Des amorces spécifiques provenant de ces séquences sont identifiées comme utiles pour l'identification de Rhizoctonia cerealis à l'aide de techniques basées sur la PCR.

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