Pcr primers for detection of legionella species and methods...

Details Pcr primers for detection of legionella species and methods... Pcr primers for detection of legionella species and methods...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 15/12 (2006.01) C07H 17/00 (2006.01) C07H 21/04 (2006.01) C12N 15/10 (2006.01) C12N 15/11 (2006.01) C12P 19/34 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2075493

2075493 9211273 PCTABS00014 This invention provides for superior nucleic acid primers for amplification of select target regions of the genome of the genus Legionella. The invention facilitates detection of pathogenic and nonpathogenic forms of this genus. The invention further provides for processes for using the primers in template dependent nucleic acid polymerase extension reactions to amplify select target regions. Kits for the use of these primers are also provided. This invention further provides for methods of controlling the intensity of visual signal for detection of duplex formation in nucleic acid hybridization assays under high stringent conditions. This method involves the blending of different capture probes onto a solid support.

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