C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/073 (2010.01) C12N 5/0735 (2010.01) A61K 35/48 (2006.01)
Patent
CA 2627645
Disclosed herein are cell cultures comprising dorsal and/or ventral PDX1- positive foregut endoderm cells and methods of producing the same. Also disclosed herein are cell populations comprising substantially purified dorsal and/or ventral PDX1-positive foregut endoderm cells as well as methods for enriching, isolating and purifying dorsal and/or ventral PDX1-positive foregut endoderm cells from other cell types. Methods of identifying differentiation factors capable of promoting the differentiation of dorsal and/or ventral PDX1- positive foregut endoderm cells, are also disclosed.
L'invention concerne des cultures cellulaires comprenant des cellules d'endoderme d'intestin antérieur dorsal et/ou ventral exprimant PDX1, et des procédés de production de celles-ci. L'invention concerne aussi des populations de cellules comprenant des cellules sensiblement purifiées d'endoderme d'intestin antérieur dorsal et/ou ventral exprimant PDX1, et des procédés visant à enrichir, à isoler et à purifier des cellules d'endoderme d'intestin antérieur dorsal et/ou ventral exprimant PDX1 par rapport à d'autres types de cellules. Des procédés d'identification de facteurs de différenciation pouvant activer la différenciation de cellules d'endoderme d'intestin antérieur dorsal et/ou ventral exprimant PDX1 sont également décrits.
Agulnick Alan
Baetge Emmanuel E.
D'amour Kevin Allen
Eliazer Susan
Cythera Inc.
Smart & Biggar
Viacyte Inc.
LandOfFree
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