Pdx1 expressing endoderm

Details Pdx1 expressing endoderm Pdx1 expressing endoderm

C - Chemistry, Metallurgy

12

N

C12N 5/073 (2010.01) C12N 5/071 (2010.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01) G01N 33/567 (2006.01)

Patent

CA 2564114

Disclosed herein are cell cultures comprising PDX1-positive endoderm cells and methods of producing the same. Also disclosed herein are cell populations comprising substantially purified PDX1-positive endoderm cells as well as methods for enriching, isolating and purifying PDX1-positive endoderm cells from other cell types. Methods of identifying differentiation factors capable of promoting the differentiation of endoderm cells, such as PDX1-positive foregut endoderm cells and PDX1-negative definitive endoderm cells, are also disclosed.

Des cultures cellulaires comprenant des cellules endodermiques positives PDX1 et leurs procédés d'obtention. Des populations de cellules comprenant des cellules endodermiques positives PDX1 sensiblement purifiées et des procédés d'enrichissement, d'isolement et de purification des cellules endodermiques positives PDX1 à partir d'autres types de cellules. Des procédés d'identification de facteurs de différenciation aptes à favoriser la différenciation des cellules endodermiques, notamment des cellules endodermiques positives PDX1 de l'intestin céphalique et des cellules endodermiques définitives négatives PDX1.

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