Peptide-based vaccine for influenza

A - Human Necessities – 61 – K

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A61K 39/145 (2006.01) C07K 14/11 (2006.01)

Patent

CA 2352454

A human synthetic peptide-based influenza vaccine for intranasal administration comprises a mixture of flagella containing at least four epitopes of influenza virus reactive with human cells, each expressed individually in Salmonella flagellin, said influenza virus epitopes being selected from the group consisting of: (i) one B-cell hemagglutinin (HA) epitope; (ii) one T-helper hemagglutinin (HA) or nucleo-protein (NP) epitope that can bind to many HLA molecules; and (iii) at least two cytotoxic lymphocyte (CTL) nucleoprotein (NP) or matrix protein (M) epitopes that are restricted to the most prevalent HLA molecules in different human populations.

Cette invention se rapporte à un vaccin peptidique synthétique humain contre la grippe, conçu pour une administration intranasale, qui comprend un mélange de flagelles contenant au moins quatre épitopes du virus de la grippe réagissant aux cellules humaines, exprimés chacun individuellement dans la flagelline de Salmonella, ces épitopes du virus de la grippe étant sélectionnés dans le groupe constitué par: (i) un épitope d'hémagglutinine (HA) de lymphocytes B; (ii) un épitope de nucléoprotéine (NP) ou d'hémagglutinine (HA) de lymphocytes T auxiliaires, qui peut se fixer à de nombreuses molécules HLA; et (iii) au moins deux épitopes de protéine matricielle (M) ou de nucléoprotéine (NP) de lymphocytes cytotoxiques (CTL), qui sont restreints aux molécules HLA les plus présentes dans différentes populations humaines.

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