Peptide or protein sequencing method and apparatus

Details Peptide or protein sequencing method and apparatus Peptide or protein sequencing method and apparatus

G - Physics

01

N

530/1, 530/3, 23

G01N 33/483 (2006.01) B01J 19/00 (2006.01)

Patent

CA 1095896

Abstract of the Disclosure A method and apparatus for the sequential degradation of protein or peptide molecules by successive coupling and cleavage reactions. Such molecules are immobilized on a macroporous reaction support surface and placed in a flowthrough reaction chamber which is mounted in a sequencer. In the Edman sequencing technique, reagents drive by pressurized inert gas are passed through the reaction chamber as follows: (a) liquid or vapor coupling reagent, (b) coupling base vapor, (c) washing solvent, (d) inert gas to partially dry the support surface (e) cleavage reagent vapor to cleave amino acid derivatives from the immobilized coupled protein or peptide chains, (f) liquid extracting solvent to withdraw the cleaved amino acid derivative. An automated sequencer to carry out said method.

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