G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/569 (2006.01) C07K 14/045 (2006.01) C07K 16/08 (2006.01)
Patent
CA 2259967
The invention relates to a peptide reagent comprising peptides immunochemically reactive with antibodies to the human cytomegalovirus (CMV). New antibodies directed to said peptides or fragments thereof are also part of the invention. Also cell lines capable of producing monoclonal antibodies are part of the invention. The invention also relates to a method for the detection of CMV or antibodies directed against CMV in a test fluid and a test kit to be used when applying the said detection methods. Detection of CMV in a test fluid or tissue specimen using antibodies, monoclonal and polyclonal, directed to said peptide, which have the characteristics of detecting both native and denatured CMV proteins are also part of said invention.
L'invention concerne un réactif de peptides comprenant des peptides immunochimiquement réactifs avec des anticorps au cytomégalovirus (CMV) humain. L'invention concerne également de nouveaux anticorps dirigés contre ces peptides ou des fragments de ces peptides, ainsi que des lignées cellulaires capables de produire des anticorps monoclonaux. L'invention concerne aussi un procédé de détection de CMV ou d'anticorps dirigés contre le CMV dans un fluide d'essai et un kit d'essai à utiliser dans l'application de ces procédés de détection. L'invention concerne enfin la détection de CMV dans un fluide d'essai ou un prélèvement tissulaire en utilisant des anticorps, monoclonaux et polyclonaux, dirigés contre ces peptides, anticorps qui ont la caractéristique de détecter des protéines de CMV endogènes et des protéines de CMV dénaturées.
Middeldorp Jaap Michiel
Van de Crommert Johannes Martinus Gerardus
Akzo Nobel Nv
Fetherstonhaugh & Co.
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