Peptide synthesis method and solid support for use in the...

C - Chemistry – Metallurgy – 07 – K

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167/44, 530/1.04

C07K 1/04 (2006.01) C08F 255/02 (2006.01) G01N 33/545 (2006.01) G01N 33/547 (2006.01)

Patent

CA 1339987

A method for the solid-phase synthesis of peptides or proteins in high yield and high purity uses a solid support consisting of a functionalized polystyrene-grafted polymer substrate, the grafted polystyrene chains being substantially non-cross-linked and having a chain molecular weight, not including optional non-reactive substitu- ents, of at least 200,000, preferably in the range of 600,000- 1,200,000 Particularly suitable polymer substrates are substrates of a polyolefin such as polyethylene The method is particularly well- suited to the compartmentalized synthesis of a multitude of peptides or proteins in a parallel and substantially simultaneous fashion. Preferred embodiments of a solid support for performing the synthesis are prepared from thin polyethylene sheet or film which has been grafted with polystyrene chains in a radical-initiated process in which the polyethylene sheet or film is immersed in a solution of optionally substituted styrene monomer in an alcohol such as methanol, the volume percentage of styrene in the solution preferably being about 30% v/v, and subjected to gamma irradiation

610012

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