C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
530/5.1, 150/15.
C07K 5/10 (2006.01) C07K 5/06 (2006.01) C07K 5/083 (2006.01) C07K 5/093 (2006.01) C07K 5/103 (2006.01) C12Q 1/37 (2006.01) C12Q 1/56 (2006.01) G01N 33/52 (2006.01)
Patent
CA 1239395
PEPTIDE-TYPE SUBSTRATES USEFUL IN THE QUANTITATIVE DETERMINATION OF ENDOTOXIN Abstract of the Disclosure Disclosed are chromogenic or fluorogenic peptide- type compounds adapted for use in determining endotoxin in a sample by a Limulus amebocyte lysate-type assay. The compounds have the general formula: R1-A1-A2-A3-A4-B-R2 wherein R1 represents hydrogen, a blocking aromatic hydrocarbon or acyl; A1 represents an L or D-amino acid selected from either Ileu, Val or Leu; A2 represents Glu or Asp; A3 represents Ala or Cyst; A4 represents Arg, B represents a linkage group selected from ester and amide linkage groups; and R2 represents a chromogenic or fluorogenic group which is covalently attached to the C-carboxyl terminal of arginine through the B linkage group and which yields a chromophoric or fluorescent marker compound of the formula R2-B-H upon enzymatic hydrolysis from the remainder of the peptide-type compound by activated LAL pro-clot enzyme. Also disclosed are methods for the determination of endotoxin in a sample by contacting the sample with a pro-clotting enzyme from Limulus amebocyte lysate and one of the above chromogenic or fluorogenic peptide-type compounds.
409962
Osler Hoskin & Harcourt Llp
Whittaker Corporation
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