Peptides and peptide-loaded antigen presenting cells for the...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/06 (2006.01) A61K 39/00 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2278189

Methods for generating antigen-specific cytotoxic T lymphocytes (CTLs) in vitro are presented in conjuntion with methods for using these CTLs for therapy in vivo. The antigen presenting cells (APCs) used herein notably include dentritic cells pretreated with GM-CSF and IL-4. Antigen-specific CTLs were obtained by incubating CD8 preparations in the presence of APCs that bound desired peptides. IL-7 was generally added at day 1 of the incubation; IL-10 was generally added one day later and during restimulation. The invention also comprises methods for using peptide pulsed dentritic cells to identify novel MHC Class I-restricted tumor antigens and subdominant epitopes.

L'invention porte sur un procédé d'obtention in vitro de lymphocytes T cytotoxiques (CTL) spécifiques d'antigènes présenté en association avec les méthodes d'utilisation in vivo desdits lymphocytes à des fins thérapeutiques. Les cellules porteuses d'antigènes (APC) utilisées dans ces travaux comportent notamment des cellules dendritiques prétraitées par du GM-CSF et de l'IL-4. Les cellules CTL spécifiques d'un antigène ont été obtenues par incubation de préparations de CD8 en présence de cellules APC qui fixent les peptides désirés. L'IL-7 était généralement ajouté au jour 1 de l'incubation, et l'IL-10 était généralement ajouté un jour plus tard, et pendant la restimulation. L'invention porte également sur des procédés d'utilisation de cellules dendritiques pulsées par peptides pour identifier des antigènes tumoraux restreints au CMH de classe I, et des épitopes subdominants.

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