Peptidyl prolyl cis-trans isomerases

Details Peptidyl prolyl cis-trans isomerases Peptidyl prolyl cis-trans isomerases

C - Chemistry, Metallurgy

12

N

C12N 15/67 (2006.01) C12N 9/20 (2006.01) C12N 9/90 (2006.01) C12N 15/61 (2006.01)

Patent

CA 2340789

The present invention provides a method for producing a secretable polypeptide in a host cell, comprising overexpressing a peptidyl prolyl cis-trans isomerase in the host cell, thereby increasing the yield of the secreted polypeptide, and a novel peptidyl prolyl cis-trans isomerase from A. niger useful in such a method.

La présente invention concerne un procédé permettant la production d'un polypeptyde pouvant être secrété dans une cellule. Ce procédé consiste en une sur-expression d'une peptidyl-prolyl-cis-trans-isomérase dans la cellule hôte. Cela permet d'augmenter la production du polypeptide secrété. L'invention concerne également une peptidyl-prolyl-cis-trans-isomérase issue de A. niger convenant à ce procédé.

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