Performing nucleic acid reactions

Details Performing nucleic acid reactions Performing nucleic acid reactions

C - Chemistry, Metallurgy

12

N

195/1.12

C12N 15/10 (2006.01) B01J 19/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 1339097

Apparatus for performing nucleic acid reactions or manipulations comprises at least one reaction vessel containing a predetermined amount of a reagent for a nucleic acid reaction in a dried state adapted to be reconstituted by addition of aqueous fluid containing a nucleic acid sequence. The reagent preferably comprises a mixture of enzyme and reaction buffer and includes all components of the reaction except the nucleic acid substrate. Reactions and manipulations exemplified include restriction, DNA modification, cDNA synthesis, and dideoxy sequencing.

571271

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