Perfusion incubator

C - Chemistry – Metallurgy – 12 – M

Patent

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Details

C12M 3/02 (2006.01) C12M 3/00 (2006.01)

Patent

CA 2400164

A perfusion incubator having a fluid conditioning unit, a well assembly and a well assembly heating unit, a peristaltic pump and a fluid collection unit. The well assembly has a number of wells each having a transparent lid and a fluid inlet and a fluid outlet. An illumination device is under the well so that the cell being cultured in the well assembly can be observed by means of a microscope. Each fluid inlet is positioned so as to allow a tangential entry of fluid to the well at a mid point in the well, and each fluid outlet is above the fluid inlet with the cell to be cultured in a lower portion of the well so that flow of fluid in the well is formed into a vortex which tends to draw fluid from around the cell without direct flow over the embryo.

Cette invention a trait à un incubateur à perfusion possédant une unité de conditionnement de fluide (12), un ensemble cupule (7) et un ensemble destiné à chauffer l'ensemble cupule (14), une pompe péristaltique (4) ainsi qu'une unité de collecte de fluide (1). L'ensemble cupule comporte un certain nombre de cupules (20, 21) pourvues, chacune, d'un couvercle transparent (30) ainsi que d'orifices d'entrée (26) et de sortie (27). Un éclairage se trouve (13) sous la cupule, ce qui permet d'observer à l'aide d'un microscope (15) les cellules en cours de culture dans l'ensemble cupule. Chaque orifice d'entrée de fluide (26) est positionné de manière à permettre une arrivée tangentielle de fluide dans la cupule au niveau d'un point médian de la cupule et chaque orifice de sortie (27) se trouve au-dessus de l'arrivée de fluide, les cellules à cultiver se trouvant dans la partie inférieure de la cupule, de sorte que l'écoulement fluidique dans la cupule forme un tourbillon tendant à attirer le fluide autour des cellules sans diriger le flux sur l'embryon.

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