Permanent amniocyte cell line, the production thereof and...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/10 (2006.01) C12N 15/34 (2006.01) C12N 15/86 (2006.01) C12N 15/861 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2391591

The invention relates to a permanent amniocytic cell line comprising at least one nucleic acid which brings about expression of the gene products of the adenovirus E1A and E1B regions. The present invention further relates to the production of a permanent amniocytic cell line and to its use for producing gene transfer vectors and/or adenovirus mutants. Further aspects are the use of amniocytes and of the adenoviral gene products of the E1A and E1B regions for producing permanent amniocytic cell lines.

L'invention concerne une lignée cellulaire permanente d'amniocytes, contenant au moins un acide nucléique provoquant l'expression des produits géniques de la région E1A et E1B d'adénovirus. L'invention concerne aussi la production d'une lignée cellulaire permanente d'amniocytes et son utilisation pour la production de vecteurs de transfert de gènes. L'invention concerne également l'utilisation d'amniocytes ou de produits géniques adénoviraux de la région E1A et E1B pour la production de lignées cellulaires permanentes d'amniocytes.

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