Permissive cells and uses thereof

Details Permissive cells and uses thereof Permissive cells and uses thereof

G - Physics

01

N

G01N 33/569 (2006.01) A61K 39/12 (2006.01) C12N 5/00 (2006.01) C12N 15/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2694476

The invention relates generally to the field of virology. More particularly, the present invention relates to methods for determining the permissiveness of a cell for a virus that is a member of the family Arteriviridae or Coronaviridae or Asf arviridae, in particular for Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). The invention further provides methods and compositions related to the generation of host cells permissive for a virus that is a member of the family Arteriviridae or Coronaviridae or Asfarviridae, in particular for Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). Methods of using said cells thus identified or thus generated, in preparing a culture of a virus that is a member of the family Arteriviridae or Coronaviridae or Asfarviridae, as well as the use of said virus for the purpose of vaccine production or diagnosis, are also provide by the present invention.

L'invention concerne en général le domaine de la virologie. L'invention concerne plus particulièrement des méthodes pour déterminer la permissivité d'une cellule à un virus membre de la famille Arteriviridae ou Coronaviridae ou Asf arviridae, en particulier au virus du syndrome respiratoire et reproducteur porcin (PRRSV). L'invention concerne également des méthodes et des compositions concernant la génération de cellules hôtes permissives à un virus membre de la famille Arteriviridae ou Coronaviridae ou Asf arviridae, en particulier au virus du syndrome respiratoire et reproducteur porcin (PRRSV). L'invention concerne enfin l'utilisation des cellules ainsi identifiées ou générées par préparation d'une culture de virus membre de la famille Arteriviridae ou Coronaviridae ou Asf arviridae, ainsi que leurs utilisations à des fins de production de vaccins ou de diagnostic.

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