Permuted and nonpermuted luciferase biosensors

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/02 (2006.01) C12N 15/53 (2006.01) C12Q 1/26 (2006.01) G01N 33/573 (2006.01) C12N 1/15 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01)

Patent

CA 2648263

A modified luciferase protein which is a sensor for molecules including cAMP, cGMP, calcium, chelators thereof, kinases, or phosphatases is provided. Also provided is a circularly permuted anthozoan luciferase protein and a decapod crustacean luciferase protein, optionally containing one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest. Further provided is a modified anthozoan luciferase protein and a decapod crustacean luciferase protein containing an insertion of one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with a molecule of interest.

La présente invention concerne une protéine luciférase modifiée qui est un capteur pour des molécules comprenant cAMP, cGMP, du calcium, des chélateurs de celles-ci, des kinases, ou des phosphatases. L'invention concerne également une protéine luciférase d'anthozoaire et une protéine luciférase de crustacé décapode, contenant éventuellement une ou des séquences d'acides aminés hétérologues, dont au moins une interagit directement ou indirectement avec une molécule d'intérêt. L'invention concerne en outre une protéine luciférase d'anthozoaire modifiée et une protéine luciférase de crustacé décapode contenant une insertion d'une ou de plusieurs séquences d'acides aminés, dont au moins une interagit directement ou indirectement avec une molécule d'intérêt.

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