Pesticidally active proteins and polynucleotides obtainable...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/32 (2006.01) A01N 63/02 (2006.01) C07K 14/195 (2006.01) C07K 14/32 (2006.01) C12N 1/20 (2006.01) C12P 19/34 (2006.01) C12P 21/02 (2006.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2486543

The subject invention provides unique biological alternatives for pest control. More specifically, the present invention relates to novel pesticidal proteins, novel sources of pesticidal proteins, polynucleotides that encode such toxins, and to methods of using these toxins to control insects and other plant pests. The subject invention relates to the surprising discovery that Paenibacillus species, and proteins therefrom, have toxicity to lepidopterans. There have been no known reports of a Paenibacillus species, strain, or protein having toxicity to lepidopterans. This is also the first known example of a Paenibacillus Cry protein that is toxic to lepidopterans. Furthermore, this is the first known report of Paenibacillus having toxin complex (TC)-like proteins. The DAS1529 isolate disclosed here is also the first known example of a natural bacterium that produces both a Cry toxin and TC proteins. The subject invention also relates to new classes of Cry and TC proteins that are pesticidally active.

La pr~sente invention concerne des alternatives biologiques uniques pour la protection phytosanitaire et, plus pr~cis~ment, de nouvelles prot~ines pesticides, de nouvelles sources de prot~ines pesticides, polynucl~otides qui sont codants pour de telles toxines, et des proc~d~s utilisant ces toxines pour contrÙler les insectes et autres phytoravageurs. Selon la pr~sente invention, on a fait la surprenante d~couverte suivante : les esp­ces <i>Paenibacilles</i> et les prot~ines qui en sont issues, sont toxiques pour les l~pidopt­res. Il n'y a eu aucun rapport sur une esp­ce <i>Paenibacille</i>, souche ou prot~ine toxique pour les l~pidopt­res. Cette invention cite le premier exemple connu d'une prot~ine Paenibacille Cry toxique pour les l~pidopt­res et fait le premier rapport connu de <i>Paenibacille</i> ayant des prot~ines de type complexe toxine (TC). L'isolat DAS1529 dont il est question ici est ~galement le premier exemple connu d'une bact~rie naturelle qui produise ~ la foisune toxine Cry et des prot~ines TC. L'invention concerne enfin de nouvelles classes de prot~ines Cry et TC ayant une action pesticide.

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