Phagocytic assay method

Details Phagocytic assay method Phagocytic assay method

C - Chemistry, Metallurgy

12

N

C12N 15/12 (2006.01) A01K 67/027 (2006.01) A61K 38/17 (2006.01) C07K 14/435 (2006.01) C07K 14/47 (2006.01) C07K 16/18 (2006.01) C12N 5/10 (2006.01) C12N 15/62 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01) G01N 33/563 (2006.01)

Patent

CA 2332993

The invention provides assay methods for determining whether a compound is an enhancer or an inhibitor of a signal transduction pathway which promotes phagocytosis of apoptotic cells. The methods involve exposing a phagocytic cell to apoptotic cells, optionally transfected with a reporter gene, and measuring the extent of phagocytosis in the presence or absence of the test compound. Expression vectors are provided to transfect mammalian cells with DNA sequences which when expressed influence the rate of phagocytosis of apoptotic cells such as the human homologue of the C. elegans ced-6 gene.

L'invention concerne des procédés d'analyse permettant de déterminer si un composé est un activateur ou un inhibiteur d'une voie de transduction de signaux, qui favorise la phagocytose de cellules en apoptose. Ces procédés consistent à exposer un phagocyte à des cellules en apoptose, éventuellement transfectées par un gène reporter, et à mesurer l'extension de la phagocytose en présence ou en l'absence du composé de test. Des vecteurs d'expression assurent la transfection de cellules de mammifère avec des séquences d'ADN, qui lorsqu'elles sont exprimées, influencent le taux de phagocytose des cellules en apoptose telles que l'homologue humain du gène ced-6 $i (C. elegans).

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