Pharmaceutical compositions containing the enzyme cyprosin,...

A - Human Necessities – 61 – K

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A61K 38/48 (2006.01) A61K 35/00 (2006.01) A61K 36/28 (2006.01) A61P 35/00 (2006.01)

Patent

CA 2700985

The object of the present invention is the use of a preparation containing a phytepsin, more specifically a cyprosin, containing the heterodimer, its N-terminal pro- peptide, the mature N-terminal peptide, and mature C-terminal peptide, as well as other precursor species, processing products, and aggregate species, either isolated or in any combinations of the former, native, extracted and partially purified from flowers of Cynara cardunculus, or recombinant, extracted from the supernatant from a culture of Saccharomyces cereviseae genetically modified for the heterologous production of cyprosin, for therapeutic applications more precisely for its use as an antitumor agent.

La présente invention concerne l'utilisation d'une préparation contenant une phytepsine, plus précisément une cyprosine, contenant l'hétérodimère, son pro-peptide N-terminal, le peptide N-terminal mature et le peptide C-terminal mature, ainsi que d'autres espèces précurseurs, produits de transformation et espèces agrégées, sous forme isolée ou en combinaison. La cyprosine native est extraite et partiellement purifiée à partir des fleurs de Cynara cardunculus, ou la cyprosine recombinante est extraite du surnageant issu d'une culture de Saccharomyces cereviseae génétiquement modifié pour produire la cyprosine de manière hétérologue. La préparation est destinée à des applications thérapeutiques, plus précisément pour un usage en tant qu'agent anticancéreux.

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