Pharmaceutical preparation for the treatment and diagnosis...

A - Human Necessities – 61 – K

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A61K 35/16 (2006.01) A61P 35/00 (2006.01)

Patent

CA 2415862

Pharmaceutical preparation for the treatment and follow-up care of tumors that comprises the blood plasma or predetermined blood plasma components of equidae animals with pair number of fingers, preferably cattle being not endangered lethally by leucosis . The predetermined fraction is the material "bovin 40" and/or "bovin 300" constituting the difference detectable by electrophoresis between the lipid-free fraction taken from a healthy cattle. In the separation method of the required fraction the initial blood is optionally treated by an anti-coagulant and the corpuscles are separated therefrom, the plasma fraction is treated by a first organic solvent, then a surfactant material composed of fine grains is added thereto, the liquid is mixed and the lipid-free fraction bound to the grains is separated from the liquid components by centrifugation, and the separated fraction is brought again into a solution.

L'invention concerne une préparation pharmaceutique destinée au traitement et au suivi de tumeurs, comprenant le plasma sanguin ou des composants prédéterminés du plasma sanguin d'animaux équidés possédant un nombre pair de doigts, de préférence du bétail qui n'est pas menacé de mort par la leucose. La fraction prédéterminée est le matériel bovin 40 et/ou bovin 300 constituant la différence décelable par électrophorèse entre la fraction exempte de lipides provenant d'un bovin atteint de leucose et la fraction exempte de lipides provenant d'un bovin en bonne santé. La méthode de séparation de la fraction requise consiste éventuellement à traiter le sang de départ avec un anticoagulant et à en séparer les corpuscules, à traiter la fraction de plasma à l'aide d'un premier solvant organique, à ajouter une matière tensioactive à grains fins, à mélanger le liquide et à séparer la fraction exempte de lipides fixée sur les grains des composants liquides par centrifugation, et à ramener dans une solution la fraction séparée.

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