Pharmacological applications of mitochondrial dna assays

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2416332

This invention provides assays to determine the relative amount of mitochondrial DNA in a subject, such as a subject undergoing drug treatment. The subject may for example be a human patient undergoing treatment for an HIV infection with a nucleic acid precursor such as a nucleoside or nucleotide analogue. The assays of the invention may include PCR assays, such semi- quantitative or quantitative PCR involving the co-amplification of a mitochondrial sequence and a reference sequence, such as a genomic sequence. Information from such assays may be evaluated to provide a ratio of mithchondrial DNA to nuclear DNA in the cells of the subject.

La présente invention concerne des analyses permettant de déterminer la quantité relative d'ADN mitochondrial chez un sujet, tel qu'un sujet sous traitement thérapeutique. Le sujet peut par exemple être un patient humain sous traitement pour une infection à VIH avec un précurseur d'acide nucléique tel qu'un nucléoside ou un analogue de nucléotide. Les analyses selon l'invention peuvent comprendre des analyses de l'amplification en chaîne par polymérase (PCR), ladite PCR semi-quantitative ou quantitative impliquant la co-amplification d'une séquence mitochondriale et d'une séquence de référence, telle qu'une séquence génomique. Des informations obtenues à partir desdites analyses peuvent être évaluées pour fournir un rapport d'ADN mitochondrial à l'ADN nucléaire présent dans les cellules du sujet.

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