Pharmacological profiling of drugs with cell-based assays

G - Physics – 01 – N

Patent

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G01N 33/53 (2006.01)

Patent

CA 2577152

The instant invention provides a method for establishing safety profiles for chemical compounds, as well as pharmacological profiling said method comprising (A) testing the effects of said chemical compounds on the amount and/or post-translational modifications of two or more macromolecules in intact cells; (B) constructing a pharmacological profile based on the results of said tests; and (C) comparing said profile to the profile(s) of drugs with established safety characteristics. Additionally, the invention is also directed to a composition comprising an assay panel, said panel comprising at least one high-content assay for the amount and/or post-~translational modification of a protein and at least one high-content assay for the amount and/or subcellular location of a protein-protein interaction.

La présente invention concerne une technique permettant d'établir des profils d'innocuité de composés chimiques ainsi que d'établir des profils pharmacologiques. Cette technique consiste (A) à tester les effets de ces composés chimiques sur la quantité et/ou des modifications post-translationnelles d'au moins deux macromolécules dans des cellules intactes, (B) à construire un profil pharmacologique fondé sur les résultats de ces tests et, (C) à comparer ce profil au(x) profil(s) des médicaments dont les caractéristiques d'innocuité sont établies. Par ailleurs, cette invention concerne aussi une composition comprenant un panneau de dosage, ce panneau comprenant au moins un dosage de grande densité de la quantité et/ou de la modification post-translationnelle d'une protéine et au moins un dosage de grande densité de la quantité et/ou de la localisation subcellulaire d'une interaction protéine-protéine.

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