Phenol-induced proteins of thauera aromatica

Details Phenol-induced proteins of thauera aromatica Phenol-induced proteins of thauera aromatica

C - Chemistry, Metallurgy

12

N

C12N 15/52 (2006.01) C12N 9/00 (2006.01)

Patent

CA 2360935

This invention pertains to genes coding for phenol-induced proteins. Five phenol-induced proteins isolated from Thauera aromatica. Three dominant phenol- induced proteins called F1, F2 and F3 respectively were purified and sequenced to obtain the enzyme(s) that catalyze the 14CO2:4-hydroxybenzoate isotope exchange reaction and the carboxylation of phenylphosphate. The N-terminal amino acid sequences of these proteins as well as the N-terminus of the phenol- induced proteins (F4 and F5) were also determined.

L'invention concerne des gènes codant pour des protéines induites par le phénol. Cinq protéines induites par le phénol sont isolées de Thauera aromatica. Trois protéines dominantes induites par le phénol appelées F1, F2, F3 sont respectivement purifiées et séquencées afin d'obtenir l'(les) enzyme(s) catalysant la réaction de substitution de l'isotope ?14¿CO¿2?:4-hydroxybenzoate et la carboxylation du phénylphosphate. Les séquences d'aminoacides N-terminaux de ces protéines ainsi que la terminaison N des protéines induites par le phénol (F4 et F5) ont également été déterminées.

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